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Free HESI A2 Biology Practice Questions (2026)

Original HESI A2 biology practice with written answers. Try the free samples below, then get all 10 biology questions in the full $39 bank.

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What the HESI A2 Biology section tests

Biology focuses on the foundations: the cell and its parts, cellular respiration and photosynthesis, metabolism, the major molecules of life, basic genetics and DNA, and how living things are classified. These are the building blocks a nursing science sequence assumes you already understand, so a clear grasp of each concept pays off later.

Read first. These are original practice questions written for study. They are not real HESI A2 exam items and are not affiliated with or endorsed by Elsevier or HESI. No nurse or clinician reviewed this content. We make no pass-rate or score guarantee.

Free Biology practice questions

1. Which organelle is the primary site of ATP production through aerobic respiration in eukaryotic cells?

A. Golgi apparatusB. MitochondrionC. LysosomeD. Rough endoplasmic reticulum
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Answer: B. Mitochondria carry out the citric acid cycle and oxidative phosphorylation, generating most of the cell's ATP.

2. A human somatic cell with 46 chromosomes undergoes mitosis. How many chromosomes will each daughter cell contain?

A. 23B. 46C. 92D. 69
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Answer: B. Mitosis produces two genetically identical diploid daughter cells, each with the same chromosome number as the parent (46).

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Part of the full 68-question HESIReady exam. One payment of $39, every section, with explanations.

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Questions people ask

Is this HESI A2 biology practice free?

Yes. These sample Biology questions are free with full explanations. The complete bank has 10 biology questions, part of the 68-question HESIReady exam for a one-time $39.

What does the HESI A2 Biology section cover?

Biology focuses on the foundations: the cell and its parts, cellular respiration and photosynthesis, metabolism, the major molecules of life, basic genetics and DNA, and how living things are classified. These are the building blocks a nursing science sequence assumes you already understand, so a clear grasp of each concept pays off later.